Mouse Immunoglobulin G1 (IgG1) ELISA Kit

Cat.#:

JL20473

Regulatory Status:

RUO

Alternative:

IGHG1; Ig Gamma-1 Chain C Region; Immunoglobulin Heavy Constant Gamma 1; G1m Marker; Immunoglobulin Gm1

Detection Method:

ELISA

Assay Type:

Competitive

Detection Range:

3.12-200ng/ml

Sensitivity:

1.34ng/mL

Specificity:

This kit recognizes Mouse IgG1 in samples. No significant cross-reactivity or interference between Mouse IgG1 and analogues was observed.

Sample Type:

Serum;plasma and other biological fluids

Assay Procedure:

Research Area:

Immunology

Size & Price:

Human A1BG Polyclonal Antibody

Email *

Message *

Please Sign In.

Product Datasheets

Instruction:

Data
Citations
Sequence / 3D

| IgG1

Immunoglobulin G (IgG) is one of the most abundant proteins in human serum, accounting for about 10–20% of plasma protein.Antibody responses to soluble protein antigens and membrane proteins primarily induce IgG1, but are accompanied with lower levels of the other subclasses, mostly IgG3 IgG4. Because IgG1 is normally the most abundant subclass, a lack of IgG1 seen in a variety of primary and secondary antibody deficiencies, can result in decreased total IgG levels (hypogammaglobulinemia). IgG1 deficiencies, sometimes in combination with other IgG subclass deficiencies, are associated with recurrent infections .

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%

| Recovery

The recovery of Mouse IgG1 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	81-101	96
Plasma(n=8)	92-105	101

| Linearity

Samples were spiked with high concentrations of Mouse IgG1 and diluted with Universal Diluent to produce samples with values within the range of the assay.

		Serum(n=4)	Plasma(n=4)
1 : 2	Range (%)	83-95	88-96
1 : 2	Average (%)	91	93
1 : 4	Range (%)	89-104	87-108
1 : 4	Average (%)	93	98